The oncogenic role of JC virus T antigen in lens tumors without cell specificity of alternative splicing of its intron.

摘要: // Wen-feng Gou 1 , Shuang Zhao Dao-fu Shen Xue-feng Yang Yun-peng Liu 2 Hong-zhi Sun Jun-sheng Luo and Hua-chuan Zheng Cancer Research Center, Key Laboratory of Brain Spinal Cord Injury Liaoning Province, Animal The First Affiliated Hospital Medical University, Jinzhou, China Department Oncological Medicine, Shenyang, Correspondence to: Zheng, email: Keywords : JC virus, T antigen, oncogenesis, transgenic mouse, lens tumor Received January 27, 2015 Accepted February 01, Published March 10, Abstract virus (JCV), a ubiquitous polyoma that commonly infects the human, is identified as etiologic agent for progressive multifocal leukoencephalopathy some malignancies. To clarify oncogenic role JCV we established two mice antigen using either α-crystallin A (αAT) or cytokeratin 19(KT) promoter. Lens tumors were found in high-copy αAT with immunopositivity p53, β-catenin N-cadherin. Enlarged eyeballs observed invaded into brain by magnetic resonance imaging hematoxylin-and-eosin staining. overall survival time homozygous was shorter than hemizygous (p<0.01), latter wild-type (p<0.01). spontaneous salivary hepatocellular carcinoma seen αAT5 no positivity antigen. KT7 suffered from lung although strongly expressed gastric epithelial cells. alternative splicing intron detectable mice, mucosa KT various cells transfected pEGFP-N1-T It suggested might induce carcinogenesis at manner cell specificity, which not linked to its intron. Both models provide good tools investigate