Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model.

摘要: Many attempts to demonstrate the oncogenic role of JC virus (JCV) have been partially successful in producing brain tumors, either by direct inoculation JCV into or transgenic models rodents. We previously reported presence DNA with a relatively high incidence pulmonary and digestive organs. However, we could not prove JCV. prepared transgene composed K19 promoter, specific bronchial epithelium T-antigen established (TG) mice. Pulmonary tumors were detected without any metastasis 2 out 15 (13.3%) 16-month-old K19/JCV TG Using immunohistochemistry (IHC), these showed T-antigen, p53 CK 19 expression, but expression nuclear cytoplasmic β-catenin insulin receptor substrate 1 (IRS1). IHC revealed same pattern as One tumor, which was examined laser capture microdissection molecular biological tools, demonstrated an EGFR mutation K-ras mutation. propose that derived from mouse model. These findings shed light on carcinogenesis.