The uteroglobin promoter targets expression of the SV40 T antigen to a variety of secretory epithelial cells in transgenic mice

摘要: Adenocarcinomas derived from the lining epithelia of various organs are most common malignant tumors in human pathology and about 50% hormone dependent. The tissue-specific hormally regulated expression rabbit uteroglobin gene is secretory epithelial cells could provide a means establishing vivo models for variety originating such tissues. We have generated trangenic mice inheriting hybrid containing 4.7 kb 5'-flanking sequences fused to SV40 T antigen encoding region. All transgenic founders examined exhibited bronchio-alveolar adenocarcinomas, probably due transgene Clara cells. Most also developed submandibular salivary gland, adenocarcinomas stomach. dysplasias male female genital tract were found single founders. Immunohistochemical analysis showed that interfered with stable maintenance differentiated phenotype as documented by endogenous gene. One founder gave rise mouse line, UT7.1. Transgenic descendants UT7.1 lung and, depending on genetic background, stomach, gland pancreas. Sporadically prostatic adenocarcinoma whereas females uterus oviduct. Thus, line be useful model several neoplasias.