作者: John Thompson , Thomas Epting , Georg Schwarzkopf , Axel Singhofen , Anne-Marie Eades-Perner
DOI: 10.1002/(SICI)1097-0215(20000615)86:6<863::AID-IJC16>3.0.CO;2-4
关键词: Pathology 、 Pylorus 、 Stomach 、 Pyloric region 、 Duodenum 、 Biology 、 Transgene 、 Genetically modified mouse 、 Carcinoembryonic antigen 、 Genetic enhancement
摘要: In an attempt to obtain suitable in vivo models for optimizing new tumor therapy strategies intestinal adenocarcinomas, carcinoembryonic antigen (CEA) promoter/SV40 T gene constructs have been used generate transgenic mice. One line (L5496), which contains a 424-bp CEA transgene, exclusively developed multi-focal carcinomas the pyloric region of stomach 100% offspring. Tumors were already observable 37-day-old animals as dysplastic cell foci within mucosal layer. 50-day-old mice, mass was mainly restricted mucosa with invasive growth into submucosal tissue. The became moribund at 100-130 days age due blockage pylorus. At this time, had penetrated duodenum and invaded all tissue layers stomach. contrast most other models, one perfectly matches development common cancers found humans. Furthermore, after crossing these mice that are human gene, double offspring revealed expression resulting tumors. Thus, well being model studying gastric carcinoma prevention, system should provide useful preclinical CEA-targeted therapy.