Transgenic mouse models for lung cancer

摘要: Transgenic technology allows the ability to target regulatory genes lungs in a cell-specific fashion. Using this technology, we have generated model investigate phenotypic consequences of targeting oncogenes particular cell types and are developing second for regulated expression lung. The transgenic involves constitutive simian virus 40 large T antigen Clara cells mouse lungs. This has been used changes cycle during transformation process, as well transcription factors regulating differentiation markers. investigates system is based on establishment two lines. regulator line consists chimeric transcriptional factor placed under control lung-specific SPC (surfactant protein C) promoter. composed activation domain, GAL4 DNA-binding truncated progesterone receptor that responsive RU 486, but not endogenous progesterone. contains silent transgene minimal promoter with upstream activating sequences (UAS) recognized by transgene. Upon breeding these lines, resulting bitransgenic mice can then be induced express only administration 486. Two generations regulators evaluated their regulate growth hormone reporter gene. demonstrated inducible distal airways