Correlation of an osteoclast antigen and ruffled border on giant cells formed in response to resorbable substrates.

摘要: The osteoclast is the specialized multinucleated cell primarily responsible for degradation of organic and inorganic components bone matrix. functional developmental relationship between osteoclasts foreign body giant cells unclear. plasma membrane ruffled border juxtaposed to surface a unique morphologic characteristic active osteoclasts. In studies reported here formation was induced in response variety materials implanted onto richly vascularized chick chorioallantoic membrane. Light electron microscopic techniques were used examine characteristics cells. addition, immunohistochemical methods demonstrate appearance 150 kD antigen on chicken recognized by monoclonal antibody 121F. Giant that formed mineralized particles exhibited borders stained positively with 121F antibody. Many hydroxyapatite possessed similar but not as extensive those observed bone. Immunohistochemical localization these suggested present, staining intensity reduced compared bone-associated mineral matrix complexes adsorption extract or osteocalcin enhanced presence elicited contrast, non-resorbable materials, such Sepharose beads, mica, methacrylate, lacked negative antigen. It appears expression correlates extent membranes Furthermore, it development are related resorbable subject extracellular dissolution. Expression this may be indicative and/or state (osteoclasts) form substrates. matrix, including osteocalcin, association mineral, lead elevated levels