作者: Songsak Kiatchoosakun , Joseph Restivo , Darryl Kirkpatrick , Brian D. Hoit
DOI: 10.1046/J.1540-8175.2002.00199.X
关键词:
摘要: OBJECTIVE M-mode echocardiography has been used extensively for estimating left ventricular (LV) mass in mice, but accuracy is limited by its unidimensional nature. This study tested whether two-dimensional (2-D) echocardiographic measurement of LV using area-length and truncated ellipsoid methods overcomes this limitation. METHODS Twenty-five anesthetized FVB/N mice (Mus musculus) underwent high resolution (15 MHZ) two-dimensionally directed 2-D echocardiography. End-diastolic epicardial endocardial areas from short-axis views the orthogonal long-axis were measured, while was calculated methods. uncorrected cube equation. Hearts removed weighed after completing studies. The correlation agreement between echocardiographic-determined gravimetric weight examined. RESULTS method correlated better with autopsy (r = 0.87, slope standard error estimate [SEE] 12.4 mg, P < 0.001) than 0.85, 0.71, SEE 11.1 0.001), degree (bias +/- 2 SD) (10.2 25.1 mg vs 13.1 25.8 NS). Although showed a good necropsy 0.78, 11.0 estimates less that mass. CONCLUSIONS an accurate determination symmetrical ventricles can be as alternative vivo mice.