Effect of Oxidizing Agents and Sulfhydryl Group Reagents on Beta Toxin from Clostridium perfringens Type C

摘要: Purified beta toxin from Clostridium perfringens type C was inactivated by the oxidizing agents o-iodosobenzoate (OIBA), oxidized glutathione, and ferricyanide, sulfhydryl group reagents 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) N-ethylmaleimide, iodoacetamide, iodoacetic acid, causing loss of activity in various degrees depending on concentration used. The not influenced exposure to 1.0 mm p-chloromercuribenzoate. treated OIBA or DTNB reactivated incubation with 2-mercaptoethanol dithiothreitol. data suggest that contains thiol groups which are essential for activity. Beta is produced only types B C. It necrotizing lethal, but hemolytic (6). thought be a main factor necrotic enteritis animals man caused We have purified extensively (11), reported heat labile protein molecular weight approximately 30,000 (12). To study role played C, it important know relationship between functional toxin. However, there been no previous reports this. previously found completely bubbling air ( J. Sakurai, Y. Fujii, M. Matsuura, unpublished data). In this paper, we report some reversibly mild oxidation.