Cartilage oligomeric matrix protein-angiopoientin-1 enhances angiogenesis of isolated islet and maintains normoglycemia following transplantation.

摘要: Islet transplantation (ITx) has potential as a therapy for patients with type 1 diabetes. For successful engraftment and insulin independence, the transplanted islets must establish an adequate, stable blood supply. Angiopoientin-1 (Ang1) is specific growth factor that induces vascularization via Tie2 or Tie1 receptor. In this study, we used in vitro angiogenesis assay to evaluate islet function following effect of Ang1 variant cartilage oligomeric matrix protein (COMP) on isolated islets. The enhanced transduced COMP-Ang1 was also confirmed streptozotocin (STZ)-induced diabetic mice model. three-dimensional collagen-based culture system, transduction into significantly increased compared bacterial-β-galactosidase (LacZ)-transduced controls intact, nontransduced negative control group. attenuated hyperglycemia syngeneic C57BL/6 glucose tolerance by areas under curves intraperitoneal tests. These findings demonstrated capacity promote revascularization cultured islets, which may contribute vivo.