Effective glycemic control achieved by transplanting non-viral cationic liposome-mediated VEGF-transfected islets in streptozotocin-induced diabetic mice.

摘要: Hypoxic damage is one of the major causes islet graft failure and VEGF known to play a crucial role in revascularization. To address effectiveness cationic lipid reagent as gene carrier, beneficial effect VEGF-transfected islets on glycemic control, we used effectene transfection experiment using mouse islets. Transfection efficiencies were highest for 4 ㎍/μL cDNA 25 μL cell viabilities also satisfactory under this condition, overproduction mRNA protein confirmed from conditioned cells. A minimal number (100 IEQ/animal) transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled transplantation (IT)-alone group (0/8) (nondiabetic glucose mice number/total recipient number) or IT-pJDK control vector (0/8). However, hyperglycemia completely abrogated IT-pJDK-VEGF transduced (8/8), viable increased grafts vascularization observed renal capsules. These studies support usefulness VEGFtransfected delivery achieve euglycemia