Assessment of islet cell viability using fluorescent dyes.

摘要: A rapid fluorometric method has been developed to evaluate the viability of isolated islet cells. The assay differentiates between viable and nonviable cells by simultaneous use inclusion exclusion dyes acridine orange propidium iodide. When viewed fluorescent microscopy, fluoresce green, while bright red. Although iodide measures membrane integrity, results this correlate with other cell viability. Compared trypan blue exclusion, is easier read, more stable, fewer staining artifacts. enables estimation a population prior time-consuming experiments rather than retrospectively. This can also be used intact islets. Stained islets divided into three distinct groups: green fluorescing contain insulin, red little or no insulin third class containing some non-viable red, yellow. yellow colour due superimposition