Mechanistic investigations into the encapsulation and release of small molecules and proteins from a supramolecular nucleoside gel in vitro and in vivo

摘要: Supramolecular gels have recently emerged as promising biomaterials for the delivery of a wide range bioactive molecules, from small hydrophobic drugs to large biomolecules such proteins. Although it has been demonstrated that each encapsulated molecule different release profile hydrogel, so far diffusion and steric impediment identified only mechanisms molecules supramolecular gels. Erosion gel not yet reported contribute profiles molecules. Here, we use novel nucleoside-based drug system proteins with properties dye describe first time how these materials interact, encapsulate eventually through an erosion-based process. Through fluorescence microscopy spectroscopy well angle X-ray scattering, show directly interact hydrogel fibres - rather than being physically entrapped in network. The ability protect against enzymatic degradation is also here time. In addition, released were proven be functional vitro. Real-time together macroscopic studies confirm erosion key mechanism. vivo, completely degrades after two weeks no signs inflammation are detected, demonstrating its vivo safety. By establishing contribution driving force behind gels, this work provides mechanistic insight into way sets basis design more tailored applications.