Therapeutic Potential of Vasculogenesis and Osteogenesis Promoted by Peripheral Blood CD34-Positive Cells for Functional Bone Healing

摘要: Failures in fracture healing are mainly caused by a lack of vascularization. Adult human circulating CD34+ cells, an endothelial/hematopoietic progenitor-enriched cell population, have been reported to differentiate into osteoblasts vitro; however, the therapeutic potential cells for is still unclear. Therefore, we performed series experiments test our hypothesis that functional supported vasculogenesis and osteogenesis via regenerative plasticity cells. Peripheral blood isolated from total mononuclear adult volunteers, showed gene expression osteocalcin 4 20 freshly single reverse transcriptase-polymerase chain reaction analysis. Phosphate-buffered saline, or were intravenously transplanted after producing nonhealing femoral fractures nude rats. Reverse immunohistochemical staining at peri-fracture site demonstrated molecular histological human-specific markers endothelial week 2. Functional bone assessed biomechanical as well radiological examinations was significantly enhanced transplantation compared with other groups. Our data suggest promote environment conducive neovascularization damaged skeletal tissue, allowing complete fractures.