Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1

摘要: The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H2 when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur terminal electron acceptor for heterotrophic growth. To gain insight into metabolism, the proteome of T. cells grown under culture conditions was quantified and compared with those H2-evolving substrate conditions. Using label-free nano-UPLC-MSE-based comparative proteomic analysis, approximately 38.4% total identified (589 proteins) found be significantly up-regulated (≥1.5-fold) Many these proteins were functionally associated carbon fixation, Fe–S cluster biogenesis, ATP synthesis, reduction, protein glycosylation, translocation, formate oxidation. Based on abundances in this other genomic studies, pathways reductive H2-metabolism, oxidative stress defense proposed. results revealed markedly lower expression levels enzymes involved assimilation pathway, well cysteine desulfurase, condition. present provide first global atlas changes triggered by sulfur, may facilitate an understanding how archaea adapt sulfur-rich, extreme environments.