Temperature Sensitivity of Sigma Background Is Suppressed by the Disruption of ScKNS1 in Saccharomyces cerevisiae
作者： Hee-Moon Park , Yun-Hee Park
DOI:
关键词:
摘要: 진핵세포 생물은 삼투압 , 열 및 산화 스트레스 등의 다양한 외부 환경의 자극을 수용하여 반응하는 신호전달경로를 가지고 있다. Saccharomyces cerevisiae의 대표적인 신호전달경로중 하나인 cAMP/PKA 경로[cAMP-dependent Protein Kinase A (PKA) pathway]는 전사조절인자인 Flo8과 Flo11를 통해 질소고갈 환경에서 균사형 생장 세포엉김 (flocculation)현상을 유도한다. Pheromone에는 Fus3, 환경에는 Kss1, 삼투 스트레스에는 Hog1, 그리고 세포벽 합성 Slt2 등, 신호에 따라 MAPK [a mitogen-activated protein (MAP) kinase pathway]로 이루어진 특성화된 보인다(4). 실험실에서 사용하는 S. cerevisiae 균주는 크게 BY4741을 포함하는 S288c 계열과 Σ1278b 계열로 구분할 수 있다 . 계열은 효모 유전체 프로젝트의 표준 균주로 일반적으로 균주이나, FLO8 유전자의 정지돌연변이(nonsense mutation)로 인하여 반수체의 부착 생장, 배수체의 위균사형 생체막 형성 형태변화가 관찰되지 않는다 (8). 그러나 환경과 에탄올 단쇄 알코올 첨가에 의해 형태변환
koreascience.or.kr 参考文章(13)
R. D. Dowell, O. Ryan, A. Jansen, D. Cheung, S. Agarwala, T. Danford, D. A. Bernstein, P. A. Rolfe, L. E. Heisler, B. Chin, C. Nislow, G. Giaever, P. C. Phillips, G. R. Fink, D. K. Gifford, C. Boone, Genotype to phenotype: a complex problem. Science. ,vol. 328, pp. 469- 469 ,(2010) , 10.1126/SCIENCE.1189015
Carlos J. Gimeno, Per O. Ljungdahl, Cora A. Styles, Gerald R. Fink, Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: Regulation by starvation and RAS Cell. ,vol. 68, pp. 1077- 1090 ,(1992) , 10.1016/0092-8674(92)90079-R
Won-Hwa Kang, Yoon-Dong Park, Ji-Sook Hwang, Hee-Moon Park, RNA-binding protein Csx1 is phosphorylated by LAMMER kinase, Lkh1, in response to oxidative stress inSchizosaccharomyces pombe FEBS Letters. ,vol. 581, pp. 3473- 3478 ,(2007) , 10.1016/J.FEBSLET.2007.06.053
Yoon-Dong Park, Won-Hwa Kang, Wan-Soo Yang, Kwang-Soo Shin, Kyung Sook Bae, Hee-Moon Park, LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast. Biochemical and Biophysical Research Communications. ,vol. 311, pp. 1078- 1083 ,(2003) , 10.1016/J.BBRC.2003.10.110
Eulàlia de Nadal, Francesc Posas, Multilayered control of gene expression by stress-activated protein kinases The EMBO Journal. ,vol. 29, pp. 4- 13 ,(2010) , 10.1038/EMBOJ.2009.346
Kun-Hoe Kim, Young-Mi Cho, Won-Hwa Kang, Ji-Hoon Kim, Kwan-Ho Byun, Yoon-Dong Park, Kyung-Sook Bae, Hee-Moon Park, Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog. Biochemical and Biophysical Research Communications. ,vol. 289, pp. 1237- 1242 ,(2001) , 10.1006/BBRC.2001.6128
Ramesh Padmanabha, Sonja Gehrung, Michael Snyder, The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family. Molecular Genetics and Genomics. ,vol. 229, pp. 1- 9 ,(1991) , 10.1007/BF00264206
Won-Hwa Kang, Yun-Hee Park, Hee-Moon Park, The LAMMER Kinase Homolog, Lkh1, Regulates Tup Transcriptional Repressors through Phosphorylation in Schizosaccharomyces pombe Journal of Biological Chemistry. ,vol. 285, pp. 13797- 13806 ,(2010) , 10.1074/JBC.M110.113555
Steffen Rupp, Eric Summers, Hsiu‐Jung Lo, Hiten Madhani, Gerald Fink, MAP kinase and cAMP filamentation signaling pathways converge on the unusually large promoter of the yeast FLO11 gene The EMBO Journal. ,vol. 18, pp. 1257- 1269 ,(1999) , 10.1093/EMBOJ/18.5.1257
Jason Ptacek, Geeta Devgan, Gregory Michaud, Heng Zhu, Xiaowei Zhu, Joseph Fasolo, Hong Guo, Ghil Jona, Ashton Breitkreutz, Richelle Sopko, Rhonda R. McCartney, Martin C. Schmidt, Najma Rachidi, Soo-Jung Lee, Angie S. Mah, Lihao Meng, Michael J. R. Stark, David F. Stern, Claudio De Virgilio, Mike Tyers, Brenda Andrews, Mark Gerstein, Barry Schweitzer, Paul F. Predki, Michael Snyder, Global analysis of protein phosphorylation in yeast Nature. ,vol. 438, pp. 679- 684 ,(2005) , 10.1038/NATURE04187