Establishment of an efficient and rapid method of multiple shoot regeneration and a comparative phenolics profile in in vitro and greenhouse-grown plants of psophocarpus tetragonolobus (L.) DC
作者： Vinayak Singh , Namita Singh Chauhan , Mohit Singh , Asif Idris , Raju Madanala
DOI: 10.4161/15592316.2014.970443
关键词:
摘要: An in vitro method of multiple shoot induction and plant regeneration Psophocarpus tetragonolobus (L.) DC was developed. Cotyledons, hypocotyls, epicotyls, internodal young seedling leaves were used as explants. MS media supplemented with various concentrations either thidiazuron (TDZ) or N6-benzylaminopurine (BAP) along NAA IAA combinations to determine their influence on induction. TDZ induced direct when epicotyls segments at 3 mg L−1 highest rate (89.2 ± 3.28%) (13.4 2.04) shoots per explant. BAP combination callus mediated cotyledons hypocotyls (2.5 mg L−1) (0.2 mg (100 2.66%) (23.2 2.66) Mature plants produced from regenerated transferred successfull...
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