The pregnant ferret as a model for studying the congenital effects of influenza virus infection in utero: infection of foetal tissues in organ culture and in vivo.

摘要: Organ cultures of ferret foetal tissues showed a similar pattern susceptibility to influenza virus that already observed for human (Rosztoczy et al., 1975); respiratory, alimentary and urogenital supported the replication but nervous lymphopoietic (those which, in man, are associated with or postnatal abnormalities) were insusceptible. In contrast corresponding tissues, placenta amnion readily viral although both umbilical cord susceptible. limited experiments, neither membranes nor susceptible became infected after intranasal inoculation pregnant ferrets various gestational ages. However, intracardial high titre (ca 10(9) EBID50) was isolated from foetuses. The at early, middle late gestation, appeared cross placental barrier infect only gestation. At this stage could be not those (respiratory, urogenital) organ culture, also small amounts which insusceptible culture (heart, tissue). Virus foetuses gestation following one hundred-fold lower dose unlike higher dose, did induce maternal febrile response. appears suitable model investigating effects on development infection it may have disadvantages regard nature strength barrier.