Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen.

摘要: Background: Many immunological techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace parasitological techniques, which are time consuming usually lack sensitivity reproducibility. Materials Methods: In this study, gigantica paramyosin (Pmy) antigen was early detected in cattle sera sandwich enzyme-linked immunosorbent assay (ELISA), evaluate Pmy performance diagnosis. This work conducted on 135 blood samples, were classified according investigation into, healthy control (30), fascioliasis (75), other parasites (30) groups. Results: The Sandwich ELISA 97.33%, specificity 95%, comparison with examination, recorded 66.66% 100% specificity, respectively. Conclusions: It clear that native F. is considered as a powerful immunodiagnosis fascioliasis, highly sensitive specific technique.