Naked eye detection of infertility based on sperm protamine-induced aggregation of heparin gold nanoparticles.

摘要: The development of an easy to use, one-pot, environmentally friendly, non-invasive and label-free colorimetric probe for the determination semen protamines, biochemical marker male fertility, using heparin gold nanoparticles (HAuNPs) is presented. affinity HAuNPs protamines was due electrostatic interactions between polycationic protamine polyanionic heparin. binding characterized by variation in plasmon absorption spectra followed a visibly observable colour change solution from red blue. We observed shift peak method exhibited linearity range 10-70 ng/mL with detection limit 5 ng/mL, which much lower than that reported sensors protamine. absorbance were highly specific presence different interfering compounds successfully applied determining real samples serum. Rather quantitative estimation, it seems provides quick screening large array positive negative and, moreover, maintains privacy user. appears be simple would very useful third-world countries where high-tech diagnostic aids are inaccessible majority population. Graphical Abstract Heparin aided visual infertility.