Effect of implantation on engineered skeletal muscle constructs.

摘要: The development of engineered skeletal muscle would provide a viable tissue for replacement and repair damaged by disease or injury. Our current tissue-engineering methods result in three-dimensional (3D) constructs that generate tension but do not advance phenotypically beyond neonatal characteristics. To develop to an adult phenotype, innervation vascularization the construct must occur. In this study, 3D were implanted into hindlimb rat, along sciatic nerve, with sural nerve isolated, transected sutured encourage innervation. Aortic ring anchors tendons biceps femoris so move dynamically endogenous muscle. After 1 week vivo, explanted, evaluated force production stained muscle, collagen markers. Implanted showed developing capillary system, epimysium-like outer layer connective increase myofibre content. beginning α-bungarotoxin clustering suggests neuromuscular junctions (NMJs) could form on given more time vivo. Additionally, increased maximum isometric from 192 ± 41 μN 549 ± 103 (245% increase) compared vitro controls, which 276 ± 23 329 ± 27μN (25% increase). These findings suggest survives implantation begins necessary interfaces needed phenotype toward However, terms production, need longer times fully phenotype.