A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages

摘要: In the present work a sensitive and accurate method by ion chromatography conductimetric detection has been developed for determination of biogenic amines in food samples at microgram per kilogram levels. The optimized extraction procedure trimethylamine, triethylamine, putrescine, cadaverine, histamine, agmatine, spermidine, spermine from real samples, as well separation conditions based on multilinear gradient elution with methanesulfonic acid use weak ionic exchange column, have provided excellent results terms resolution efficiency. Extended calibration curves (up to 200 mg/kg, r > 0.9995) were obtained all analyzed compounds. gave limits range 23–65 μg/kg quantification spiked blank 65–198 μg/kg. Recovery values ranged 82 103 %, amines, good repeatability was precision levels 0.03–0.32 % (n = 4). feasibility potential tested analysis such tinned tuna fish, anchovies, cheese, wine, olives, salami.