Nucleotide sequence of the tra YALE region from IncFV plasmid pED208.

摘要: Abstract The pED208 plasmid is a 90-kilobase conjugative which the derepressed form of Fo lac (IncFV). A 3.3-kilobase HindIII-PstI fragment from was cloned and sequenced found to contain four open reading frames were highly homologous traA, traL, traE, traY gene products F plasmid. The traA propilin protein 119 amino acids in length, consisting leader sequence 55 mature pilin subunit 64 residues. contained hydrophobic region followed by classic signal peptidase cleavage site (Ala-Ser-Ala-55). proteins shared 27 conserved residues had similar predicted secondary structures. traL genes separated single base pair, no ribosome binding preceded gene. an IS2 insertion element orientation II 180 nucleotides (60 residues) upstream stop codon. This resulted fusion peptide 69 for may provide observed activity. Since absent wild-type plasmid, lac, derepression concomitant multipiliation be due providing constitutive expression tra operon.