Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon
作者： David V. Thompson , Leo S. Melchers , Ken B. Idler , Rob A. Schilperoort , Paul J.J. Hooykaas
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摘要: The complete nucleotide sequence of the virB locus, from octopine Ti plasmid Agrobacterium tumefaciens strain 15955, has been determined. In large virB-operon (9600 nucleotides) we have identified eleven open reading frames, designated virB1 to virB11. From DNA analysis it is proposed that nearly all VirB products, i.e. VirB1 VirB9, are secreted or membrane associated proteins. Interestingly, both a protein (VirB4) and potential cytoplasmic (VirB11) contain consensus amino acid ATP-binding view conjugative T-DNA transfer model, proteins suggested act at bacterial surface there play an important role in directing plant cells.
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