Characterization of CD44-Mediated Cancer Cell Uptake and Intracellular Distribution of Hyaluronan-Grafted Liposomes
作者： Hussaini Syed Sha Qhattal , Xinli Liu
DOI: 10.1021/MP2000428
关键词:
摘要: Hyaluronan (HA) is a biocompatible and biodegradable linear polysaccharide which of interest for tumor targeting through cell surface CD44 receptors. HA binds with high affinity to receptors, are overexpressed in many tumors involved cancer metastasis. In the present study, we investigated impact molecular weight (MW), grafting density, receptor density on endocytosis HA-grafted liposomes (HA–liposomes) by cells. Additionally, intracellular localization HA–liposomes was determined. HAs different MWs (5–8, 10–12, 175–350, 1600 kDa) were conjugated varying degrees density. quantified using hexadecyltrimethylammonium bromide turbidimetric method. Cellular uptake subcellular evaluated flow cytometry fluorescence microscopy. Mean particle sizes ranged from 120 180 nm increased increasing size HA. HA–liposome upta...
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