Aspergillus fumigatus proteases, Asp f 5 and Asp f 13, are essential for airway inflammation and remodelling in a murine inhalation model

摘要: BACKGROUND: In susceptible individuals, exposure to Aspergillus fumigatus can lead the development of atopic lung diseases such as allergic bronchopulmonary aspergillosis (ABPA) and severe asthma with fungal sensitization (SAFS). Protease allergens including Asp f 5 13 from fumigatus, are thought be important for initiation progression asthma. OBJECTIVE: To assess importance secreted protease (matrix metalloprotease) (serine protease) in induced inflammation, airway hyperactivity, atopy wall remodelling a murine model following chronic allergens. Methods: BALB/c mice were repeatedly intranasally dosed over course five weeks culture filtrate wild-type (WT), null (?5) or (?13) strains fumigatus. Airway hyperreactivity was measured by non-invasive whole-body plethysmography, Th2 response inflammation ELISA cell counts, whilst assessed histological analysis. RESULTS: Parent WT ?5 filtrates showed high activity, activity ?13 low. Chronic intranasal three different led comparable response. However, allergen deleted strains, particular filtrate, significantly less compared filtrate. CONCLUSION: proteases, 13, recruitment inflammatory cells airways this model. deletion single fails alleviate immune Targeting conditions SAFS ABPA may have beneficial effects preventing key aspects pathology. This article is protected copyright. All rights reserved.