Decreased neutralizing antigenicity in IBV S1 protein expressed from mammalian cells.
作者： Kiyohiko Andoh , Kiyotaka Suenaga , Masashi Sakaguchi , Kenichi Yamazaki , Takashi Honda
DOI: 10.1016/J.VIRUSRES.2015.06.019
关键词:
摘要: We evaluated the antigenicity of recombinant infectious bronchitis virus (IBV) S1 protein expressed in mammalian cells. Recombinant was as a secreted fused with trimerization motif peptide, then purified using Ni Sepharose. The analyzed by Western blotting, mixed oil adjuvant, and administered to 29-day-old specific-pathogen-free chickens. Six weeks after immunization, anti-IBV neutralizing titer anti-S1 ELISA were determined; immunized chickens inoculated IBV via trachea ciliary activity observed. Results showed that highly glycosylated, lower than inactivated virus. However, indicated induced antibodies against S1. These results suggest may retain non-neutralizing epitopes but have unnatural glycosylation pattern conformation, resulting lacking conformational epitopes. In conclusion, from cells decreased, not sufficient induce antibodies.
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