摘要: Genome editing represents a group of newly developed capabilities that allow nucleotide-level targeting genomic regions in wide range species. This chapter provides an overview these technologies which open the possibility creating directed mutations virtually any location genome. functions through use engineered endonucleases create double-strand DNA breaks targeted locus. repair mechanisms, either nonhomologous end joining or homologous recombination, leave desired genetic modifications loci. Engineered genome allows knockout and knock-in experiments to be carried out species collection one-cell embryos their subsequent transfer is possible, eliminating necessity using embryonic stem cell intermediates. We introduce ZFN, TALEN, homing endonuclease, Cas/CRISPR techniques together with listing resources suppliers assist design, construction, carrying engineering experiments.
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