Preparation of a new C18 stationary phase containing embedded urea groups for use in high-performance liquid chromatography

摘要: Abstract A new C 18 urea stationary phase was prepared by a single-step modification process through the reaction of ProntoSil spherical silica (3 μm, Bischoff) with trifunctional alkoxysilane (CH 3 CH 2 O) –Si–(CH ) –NH–C(O)–NH–(CH 17 –CH , in our laboratory. The characterized elemental analysis and solid-state 29 Si 13 nuclear mangnetic resonance spectrometry. Chromatographic evaluations 150×3.9 mm HPLC columns involving separation different test mixtures, indicate good performance for both polar basic mixtures also show promising results further applications.