Quantitative profiling of epoxyeicosatrienoic, hydroxyeicosatetraenoic, and dihydroxyeicosatetraenoic acids in human intrauterine tissues using liquid chromatography/electrospray ionization tandem mass spectrometry.
作者： Ji-Hong Zhang , Timothy Pearson , Balwir Matharoo-Ball , Catharine A. Ortori , Averil Y. Warren
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摘要: Abstract A reversed-phase liquid chromatography negative ion electrospray tandem mass spectrometry (LC/ESI–MS/MS) method was developed and validated to quantify a range of physiologically relevant eicosanoids, including 5,6-, 8,9-, 11,12-, 14,15-epoxyeicosatrienoic acids (EETs); 5-, 8-, 9-, 12-, 15-hydroxyeicosatetraenoic (HETEs), 8,15-, 12,20-dihydroxyeicosatetraenoic (DiHETEs) in human intrauterine tissues. solid-phase extraction employed extract the gradient LC separation performed on Kromasil C 18 column. Mass spectrometric detection by multiple reaction monitoring over 31-min run time. The calibration curves were linear 4–400 pmol/g tissue, intra- interday precision accuracy within coefficient variation 2.0 27.4% 4.6 17.9%, respectively. lower limit quantitation 1.0 pmol/g tissue. applied successfully characterization eicosanoids different compartments Our results demonstrate significantly greater amounts HETEs than either EETs or DiHETEs ( P ρ = −0.390,
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