Cloning of fibrinolytic enzyme gene from Bacillus subtilis isolated from Cheonggukjang and its expression in protease-deficient Bacillus subtilis strains.

摘要: Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A enzyme gene, aprE2, encoding a 29 kDa cloned genomic DNA of CH3-5, sequence determined. aprE2 overexpressed in heterologous B. strains deficient extracellular using E. coli-Bacillus shuttle vector. AprE2 band observed seemed exhibit higher activities towards fibrin rather than casein.