Development of a RAPD-PCR method for identification of Bacillus species isolated from Cheonggukjang.

摘要: A RAPD-PCR (Randomly Amplified Polymorphic DNA-PCR) method was developed for rapid identification of Bacillus species, especially B. subtilis, licheniformis, and amyloliquefaciens, the most frequently isolated organisms from fermented soy foods such as Cheonggukjang, a Korean traditional food. using 10-mer (S-30) produced species specific bands reproducibly. All subtilis strains tested common 0.5 0.88 kb in size. amyloliquefaciens generated 1.1 1.5 together with fragment whereas licheniformis 1.25, 1.70, 1.9 an occasional band. Using protocol, six bacilli Cheonggukjang were identified to level, which difficult by 16S rRNA gene recA sequencing some isolates. The fragment, major band strains, internal part ytcP encoding hypothetical ABC-type transporter. primer pair designed based on sequences PCR 0.46 only strains.