Morphological and molecular identification of Explanatum explanatum in domestic water buffalo in Pakistan

摘要: More than 70 species of the family Paramphistomatidae, have been identified in ruminants different parts world. Most are pathogenic, causing amphistomosis. Adult flukes this Family a predilection for rumen, liver or bile duct where they may cause damage to epithelium. Identification adult paramphistomes level based on morphology alone requires specialized knowledge, whereas, molecular genetic marker analysis is more precise and transferable. In present study, we performed both morphological characterization fifteen collected from domesticated buffalo Punjab province Pakistan. The five these was examined detail basis were as either Explanatum explanatum bathycotyle. PCR sequencing ITS-2 rDNA region 5 flukes, plus 10 others, revealed single haplotype all cases. This differed by just nucleotide polymorphism previously described E. sequence. Phylogenetic comparison ITS2-rDNA sequences with those other Fasciola Dicrocoelium assess within between variation validate use robust species-specific explanatum. work provides validated first report parasite