Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus).

摘要: Background and Aim Paramphistomiasis is common in tropical countries such as Indonesia affects livestock various endemic wild animals Sumatran elephants. However, the specific species of paramphistomoid worm that causes paramphistomiasis are rarely reported. The study aims at identifying infects Materials Methods Flukes were collected from feces five semi-captive elephants lived Tegal Yoso Elephant Response Unit Way Kambas National Park, 2018, after treatment oxyclozanide 1 g dose approximately 5-8 mg/kg body weight. Eight worms flattened stained Semichon's carmine for morphological identification, other used molecular identification second internal transcribed spacer (ITS-2) ribosomal deoxyribonucleic acid sequence. Results Forty-five flukes Lampung, Indonesia. morphologically identified Pfenderius heterocaeca isolates did not show any variation ITS-2. Phylogenetic analysis showed there was a close genetic relationship between our sample Chiorchis fabaceus had family similar to samples. Conclusion Based on characteristics, paramphistomoids found elephant Park P. heterocaeca.