Methylation of the first exon in the erythropoietin receptor gene does not correlate with its mRNA and protein level in cancer cells

摘要: Erythropoietin receptor (EPOR) is a functional membrane-bound cytokine receptor. (EPO) represents an important hematopoietic factor for production, maturation and differentiation of erythroid progenitors. In non-hematopoietic tissue, EPO/EPOR signalization could also play cytoprotective anti-apoptotic role. Several studies identified pro-stimulating effects in tumor cells; however, numerous opposed this fact due to the usage unspecific EPOR antibodies thus potential absence or very low levels cells. It seems that problem more complex therefore we have decided focus on expression at several such as role methylation regulation expression, identification possible transcripts presence protein selected Methylation status was analysed by bisulfite conversion reaction, PCR sequencing. The monitored quantitative RT-PCR western blot analysis. study investigated exon 1 gene human cancer cell lines. Our results indicated CpGs do not significant transcription. However, type dependent. We observed existence two splice variants ovarian adenocarcinoma line - A2780 confirmed these cells using specific A82 anti-EPOR antibody. outlined all lines benefit future investigations. Moreover, antibody our previous demonstrating