Evidence of the role of protein kinase C during aclacinomycin induction of erythroid differentiation in K562 cells
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DOI: 10.1016/S0014-5793(99)00838-8
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摘要: Abstract At subtoxic concentrations, aclacinomycin is effective in controlling erythroid differentiation of K562, a human erythroleukemic cell line. To better understand early events implicated this process, we have used bisindolylmaleimide (GF109203X), an inhibitor with high selectivity for protein kinase C (PKC). Our data show that GF109203X inhibits effects on evidenced by strong reduction hemoglobinized cells and marked decrease mRNA rates genes. establish firmly PKC involvement, also verified stimulates its rapid translocation, from the cytosolic to membrane compartment. By Western blot analysis, after short induction times, PKCα was most implicated.
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sci-hub.se 参考文章(26)
Patel Hr, Sytkowski Aj, Erythropoietin activation of AP1 (Fos/Jun). Experimental Hematology. ,vol. 23, pp. 619- 625 ,(1995)
Ushio Kikkawa, Ryoji Minakuchi, Yoshimi Takai, Yasutomi Nishizuka, [31] Calcium-activated, phospholipid-dependent protein kinase (protein kinase C) from rat brain Methods in Enzymology. ,vol. 99, pp. 288- 298 ,(1983) , 10.1016/0076-6879(83)99064-X
N.R. Murray, G.P. Baumgardner, D.J. Burns, A.P. Fields, Protein kinase C isotypes in human erythroleukemia (K562) cell proliferation and differentiation. Evidence that beta II protein kinase C is required for proliferation. Journal of Biological Chemistry. ,vol. 268, pp. 15847- 15853 ,(1993) , 10.1016/S0021-9258(18)82331-6
Nathalie Belhacène, Laurence Maulon, Sandrine Guérin, Jean Ehrland Ricci, Bernard Mari, Yves Colin, Jean Pierre Cartron, Patrick Auberger, Differential expression of the Kell blood group and CD10 antigens: two related membrane metallopeptidases during differentiation of K562 cells by phorbol ester and hemin The FASEB Journal. ,vol. 12, pp. 531- 539 ,(1998) , 10.1096/FASEBJ.12.7.531
D. Toullec, P. Pianetti, H. Coste, P. Bellevergue, T. Grand-Perret, M. Ajakane, V. Baudet, P. Boissin, E. Boursier, F. Loriolle, The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. Journal of Biological Chemistry. ,vol. 266, pp. 15771- 15781 ,(1991) , 10.1016/S0021-9258(18)98476-0
Dwight M. Morrow, Mark L. Tykocinski, Barbara A. Hocevar, Alan P. Fields, Protein kinase C isotypes in human erythroleukemia cell proliferation and differentiation. Journal of Cell Science. ,vol. 101, pp. 671- 679 ,(1992)
P. Jeannesson, R. Lahlil, B. Chenais, L. Devy, R. Gillet, A. Aries, F. Morceau, C. Trentesaux, Anthracyclines as tumor cell differentiating agents: effects on the regulation of erythroid gene expression. Leukemia & Lymphoma. ,vol. 26, pp. 575- 587 ,(1997) , 10.3109/10428199709050893
Gayle E. Woloschak, Is PKC activation required for leukemia cell differentiation Leukemia Research. ,vol. 21, pp. 411- 414 ,(1997) , 10.1016/S0145-2126(96)00121-X
R. Mantovani, N. Malgaretti, S. Nicolis, A. Ronchi, B. Giglioni, S. Ottolenghi, The effects of HPFH mutations in the human γ-globin promoter on binding of ubiquitous and erythroid specific nuclear factors Nucleic Acids Research. ,vol. 16, pp. 7783- 7797 ,(1988) , 10.1093/NAR/16.16.7783
Lodewijk V. Dekker, Peter J. Parker, Protein kinase C - a question of specificity Trends in Biochemical Sciences. ,vol. 19, pp. 73- 77 ,(1994) , 10.1016/0968-0004(94)90038-8