Fast ribozyme cleavage releases transcripts from RNA polymerase II and aborts co-transcriptional pre-mRNA processing.

作者: Nova Fong , Marie Öhman , David L Bentley

DOI: 10.1038/NSMB.1652

关键词:

摘要: … splicing defects caused by ribozyme cutting in our experiments would be underestimated if unspliced precursors are less stable than spliced … ribozyme 21 , so we compared splicing with …

参考文章(47)
D. A. Samarsky, G. Ferbeyre, E. Bertrand, R. H. Singer, R. Cedergren, M. J. Fournier, A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 6609- 6614 ,(1999) , 10.1073/PNAS.96.12.6609
Frank Rigo, Amir Kazerouninia, Anita Nag, Harold G. Martinson, The RNA tether from the poly(A) signal to the polymerase mediates coupling of transcription to cleavage and polyadenylation. Molecular Cell. ,vol. 20, pp. 733- 745 ,(2005) , 10.1016/J.MOLCEL.2005.09.026
Mariangela Morlando, Monica Ballarino, Natalia Gromak, Francesca Pagano, Irene Bozzoni, Nick J Proudfoot, Primary microRNA transcripts are processed co-transcriptionally. Nature Structural & Molecular Biology. ,vol. 15, pp. 902- 909 ,(2008) , 10.1038/NSMB.1475
M. J. Mortillaro, B. J. Blencowe, X. Wei, H. Nakayasu, L. Du, S. L. Warren, P. A. Sharp, R. Berezney, A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 8253- 8257 ,(1996) , 10.1073/PNAS.93.16.8253
Andrea Újvári, Donal S. Luse, Newly initiated RNA encounters a factor involved in splicing immediately upon emerging from within RNA polymerase II Journal of Biological Chemistry. ,vol. 279, pp. 49773- 49779 ,(2004) , 10.1074/JBC.M409087200
Michael J. Dye, Natalia Gromak, Nick J. Proudfoot, Exon tethering in transcription by RNA polymerase II. Molecular Cell. ,vol. 21, pp. 849- 859 ,(2006) , 10.1016/J.MOLCEL.2006.01.032
Susan M. Berget, Exon Recognition in Vertebrate Splicing Journal of Biological Chemistry. ,vol. 270, pp. 2411- 2414 ,(1995) , 10.1074/JBC.270.6.2411
Andrea Kyburz, Arno Friedlein, Hanno Langen, Walter Keller, Direct Interactions between Subunits of CPSF and the U2 snRNP Contribute to the Coupling of Pre-mRNA 3′ End Processing and Splicing Molecular Cell. ,vol. 23, pp. 195- 205 ,(2006) , 10.1016/J.MOLCEL.2006.05.037