Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using the XT-2000iV analyzer.

摘要: Background Changes in canine hematology measurements may occur when analyses are delayed due to shipment of specimens a laboratory. Objective The purpose this study was report changes hematologic variables healthy and diseased blood measured with Sysmex XT-2000iV during storage at room temperature for 24 48 hours. Methods EDTA-K3 samples from 42 dogs were on analyzer within one hour sampling, after 48 hours the dark. Results Storage caused little or no change RBC count, HGB concentration MCH, while there moderate increase HCT, MCV reticulocytes decrease MCHC. Decreased platelet counts by impedance (PLT-I) optical (PLT-O) associated increased mean volume (MPV), platelet-large cell ratio (P-LCR) distribution width (PDW), including right shift histogram dispersion dot plot scattergram. The total differential WBC count remained stable except decreased monocyte counts. In scatterplots, monocytes shifted into lymphocyte population 24 hours, neutrophil appearing eosinophil gate storage. disease status had only small effect storage-induced changes, observed consequences clinical decisions. Conclusions Blood accompanied variations some variables, awareness which helps avoiding misinterpretations.