Characterisation of taeniid cestodes by DNA analysis.
作者： D.P. MACMANUS
DOI: 10.20506/RST.9.2.500
关键词:
摘要: This review describes how the application of molecular technology is providing improved methods for characterising and identifying taeniid cestodes. Emphasis has been placed on use DNA approaches specific diagnosis various life-cycle stages, unambiguous species strain discrimination, determination evolutionary phylogenetic relationships assignment function to key macromolecules based inferred amino acid sequences corresponding cloned cDNA sequences. In addition, isolating from taeniids, cloning genomic mitochondrial these organisms characterisation egg detection. Lastly, focuses polymerase chain reaction (PCR) which can provide basis exquisitely sensitive assays diagnostic individual species. Coupled with direct sequencing techniques, PCR also a valuable new method examining genetic variation between within
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