COMPARISON OF REACTIONS OF ANTIBODIES TO RAT COLLAGEN AND TO RAT KIDNEY IN THE BASEMENT MEMBRANES OF RAT RENAL GLOMERULI

摘要: By in vivo and vitro methods of immunofluorescence, antibody to rat collagen kidney show the same regular, linear fluorescence following outlines renal glomerular capillaries. Absorption each antiserum with its homologous antigen completely removed for while absorption heterologous had no effect. The nephrotoxicity persisted anti-kidney serum absorbed collagen. pretreatment frozen normal sections various enzymes followed by it was shown that trypsin hyaluronidase effect on subsequent either antibody; papain reduced fluorescence; pepsin Pronase acted both antigens so present. One preparation neuraminidase, derived from V. cholerae, antibodies some preparations, but enzyme influenza virus or C. perfringens either. Collagenase prevented kidney. findings this study is directed basement membranes reacts other than these membranes.