Antigenicity of rat collagen. Demonstration of antibody to rat collagen in the renal glomeruli of rats by fluorescence microscopy.

摘要: Rabbit serum or globulin, containing antibody to rat collagen, injected intravenously intracardially into normal adjuvant-prepared rats becomes fixed in the basement membranes of renal glomeruli and, a slight extent, tubules. When examined by ultraviolet light, this can be identified tissue sections yellow-green fluorescence occurring where rabbit associated with collagen antibody, has reacted fluorescein-conjugated anti-rabbit globulin from ducks. The reaction its antigen kidney is primary factor production glomerular injury which occurs prepared adjuvant. Adjacent control failed fluoresce when pretreated unlabeled treated heterologous conjugated anti-duck rabbits. remains as long 92 days and been detected early 45 minutes after injection. serum, anti-fish anti-rat absorbed was substituted for could not demonstrated fluorescence; but absorption fish did affect fixation. This series immunologic tests indicates that anti-collagen reacts homologous antigen, presumably tubules, specific used identify other tissues animal body.