Metal composition analysis of hydrogenase from Thiocapsaroseopersicina by proton induced X-ray emission spectroscopy
作者： Cs. Bagyinka , Z. Szokefalvi-Nagy , I. Demeter , K.L. Kovacs
DOI: 10.1016/0006-291X(89)92014-7
关键词:
摘要: Polyacrylamide gel electrophoresis combined with proton induced X-ray emission spectroscopy is suitable to identify and determine the relative amounts of protein bound metals in situ. An analysis hydrogenase from Thiocapsa roseopersicina has shown feasibility technique provides new insight into amount as well intramolecular location Fe Ni metal atoms this enzyme.
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