AN IN VITRO SYSTEM FOR THE STUDY OF THE MECHANISM OF ANTIGENIC STIMULATION IN THE SECONDARY RESPONSE.

摘要: The uptake of [14C]thymidine by spleen cell suspensions from rabbits previously immunized to heterologous serum proteins is stimulated the addition antigen in vitro. This stimulation specific and dependent upon concentration antigen. It represents an increased rate DNA synthesis division certain cells suspension. These are large undifferentiated mononuclear comprising approximately 1 per cent total population. suggested that this response vitro counterpart cellular proliferation seen whole animals following re-injection procedure described provides a quantitative system for study mechanism antigenic stimulation. Some preliminary experiments reported.