Prevention of glutaraldehyde-fixed bioprosthetic heart valve calcification by alcohol pretreatment: further mechanistic studies.

摘要: BACKGROUND AND AIM OF THE STUDY Calcification is a major cause of failure bioprosthetic heart valves derived from glutaraldehyde-crosslinked bovine pericardium or porcine aortic valve (PAV) cusps. Recently, we have shown that ethanol pretreatment PAV cusps prevents calcification in animal models. METHODS RESULTS In this study showed was equally effective preventing (control Ca2+ = 121.16+/-7.49 microg/mg tissue; ethanol-pretreated 2.95+/-0.78 microg/mg). Furthermore, other low-molecular weight alcohols such as methanol and isopropanol were also mitigating Storage glutaraldehyde before implantation allowed partial return calcification, suggesting role for ethanol-glutaraldehyde interactions calcification. However, when stored ethanolic up to one month, the anti-calcification effect persisted. The conditions whereby crosslinked pure, non-aqueous, alcoholic solutions examined. crosslinking equivalent standard aqueous indicated by thermal denaturation temperatures (Td) obtained differential scanning calorimetry (DSC) resistance collagenase digestion. these had lower water content marked decrease spin-lattice relaxation times (T1) solid-state proton nuclear magnetic resonance (NMR). Moreover, calcified heavily 21-day rat subdermal implants. Thus, alcohol treatment during not useful. CONCLUSION Glutaraldehyde storage after aggravates calcification; moreover, alcoholic-glutaraldehyde are beneficial anti-calcification. Ethanol glutaraldehyde-pretreated its