Enhancing repair of full-thickness excisional wounds in a murine model: Impact of tissue-engineered biological dressings featuring human differentiated adipocytes.

摘要: Promotion of skin repair for acute or chronic wounds through the use tissue-engineered products is an active field research. This study evaluates effects mediated by biological dressings containing human in vitro-differentiated adipocytes and adipose-derived stromal cells (ASCs). Re-epithelialization, granulation tissue formation neovascularization full-thickness cutaneous were specifically assessed using a murine model featuring fluorescent epidermis. In comparison with that did not receive adipocyte-containing dressing, treated displayed slight but significantly faster wound closure based on macroscopic observations over 18 days. Non-invasive imaging GFP-expressing keratinocytes determined kinetics re-epithelialization similar both groups. Treated featured thicker tissues (1.7-fold, P < 0.0001) enriched collagens (1.3-fold, 0.0104). addition, cryosections labeled detection CD31-expressing indicated 2.2-fold (P 0.0002) increased at time terminal biopsy. accordance secretion pro-angiogenic factors detected media conditioned dressings. Taken together, these results establish new type engineered substitutes mixture ASCs can promote healing when applied as temporary dressings, suggesting their potential relevance management studies.