Life history and functional capacity of the microbiome are altered in beta-cypermethrin-resistant cockroaches.

摘要: Cockroaches are widely perceived to evolve resistance insecticides. Over-expression of a resistance-conferring gene can be costly and may require energy resource reallocation for metabolic developmental processes. To evaluate whether changes in the composition gut microbiota Blattella germanica affected its evolution beta-cypermethrin determine role host growth development, we studied relationship between insecticide diversity genetic content cockroaches. Results suggest beta-cypermethrin-resistant cockroaches (R strain) exhibited delayed development period reduced adult longevity compared with susceptible (S strain). Based on 16S rRNA sequencing community metagenomics, found that relative abundance Lactobacillus Acetobacteraceae were significantly lower R strain S foregut midgut both strains. Functional annotation Kyoto Encyclopedia Genes Genomes (KEGG) modules genes two strains revealed 10.6% involved metabolism, while was 7.4%. Unigenes also translated into amino acid sequences assigned protein families based hits Carbohydrate-Active enzymes (CAZy) database. This process identified glycoside hydrolases, glycosyl transferases carbohydrate-binding as all being higher than those strain. Overall, conclude fitness-related costs increased resistant strain, variation insect microbiota, especially related an important influencing factor.