Genomic analysis reveals MATH gene(s) as candidate(s) for Plum pox virus (PPV) resistance in apricot (Prunus armeniaca L.)

摘要: Summary Sharka disease, caused by Plum pox virus (PPV), is the most important viral disease affecting Prunus species. A major PPV resistance locus (PPVres) has been mapped to upper part of apricot (Prunus armeniaca) linkage group 1. In this study, a physical map PPVres in PPV-resistant cultivar ‘Goldrich’ was constructed. Bacterial artificial chromosome (BAC) clones belonging resistant haplotype contig were sequenced using 454/GS-FLX Titanium technology. Concurrently, whole genome seven varieties (three and four PPV-susceptible) two PPV-susceptible relatives (P. sibirica var. davidiana P. mume) obtained Illumina-HiSeq2000 platform. Single nucleotide polymorphisms (SNPs) within interval, recorded from alignments against peach genome, allowed us narrow down region ∼196 kb. Searches for linked coupling with led identification 68 variants 23 predicted transcripts according annotation. Candidate genes ranked combining data variant calling functions inferred sequence homology. Together, results suggest that members cluster meprin TRAF-C homology domain (MATHd)-containing proteins are likely candidate apricot. Interestingly, MATHd hypothesized control long-distance movement (LDM) potyviruses Arabidopsis, restriction LDM also component Although gene(s) remains be unambiguously identified, these pave way determination underlying mechanism development more accurate breeding strategies.