First-trimester fetal sex determination in maternal serum using real-time PCR

摘要: An Erratum has been published for this article in Prenatal Diagnosis 22(13) 2002, 1241. Fetal sex prediction can be achieved using PCR targeted at the SRY gene by analysing cell-free fetal DNA maternal serum. Unfortunately, results reported to date show a lack of sensitivity, especially during first trimester pregnancy. Therefore, determination serum analysis could not replace karyotype following chorionic villus sampling. A new highly sensitive real-time was developped detect an sequence Analysis performed on 121 pregnant women pregnancy (mean gestational age: 11.8 weeks). Among them, 51 had least one previous male-bearing Results were compared with sex. SRY complete concordance sex. women, 61 bearing male fetus and 60 a female fetus. No false-negative observed. Furthermore, no false-positive occurred, even though 27 carrying current pregnancy. This study demonstrates that reliable, non-invasive This approach should have great implications management who are carriers X-linked genetic disorder. diagnosis might thus fetuses only, avoiding invasive procedures risk loss fetuses. Copyright © 2001 John Wiley & Sons, Ltd.