Stimulation of Release and Synthesis of Luteinizing Hormone (LH) and Follicle Stimulating Hormone (FSH) in Tissue Cultures of Rat Pituitaries in Response to Natural and Synthetic LH and FSH Releasing Hormone

摘要: Addition of nanogram amounts highly purified or pure porcine LH-RH/FSH-RH, once daily for 5 days to female rat anterior pituitary cultures, significantly increased the release both LH and FSH. appeared be linearly related log-dose LH-RH/FSHRH over range S-40 ng/ml/day. Total content FSH in stimulated tissue medium exceeded that controls as measured by radioimmunoassays Synthetic LH-RH/FSH-RH also synthesis cultures. This indicates releasing activities are intrinsic same molecule. When was incubated with tritiated glucosamine presence there a significant incorporation radioactivity into found incubation medium. These data indicate de novo synthesis, well LH, occurred LH-RH/FSH-RH. (Endocrinology 90: 764,...