The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms

摘要: The influence of a novel, safe antibiofilm therapy on the mechanical properties Pseudomonas aeruginosa and Acinetobacter baumannii biofilms in vitro was characterized. A multiscale approach employing atomic force microscopy (AFM) rheometry used to quantify disruption by therapeutic polymer based low-molecular weight alginate oligosaccharide (OligoG). AFM demonstrated structural alterations exposed OligoG, with significantly lower Young's moduli than untreated biofilms, (149 MPa vs 242 MPa; p < 0.05), decreased resistance hydrodynamic shear an increased surface irregularity (Ra) controls (35.2 nm ± 7.6 12.1 5.4; 0.05). Rheology that increasing clinically relevant concentrations OligoG (<10%) were associated phase angle (δ) over wide range frequencies (0.1-10 Hz). These results highlight utility these techniques for study three-dimensional quantifying novel therapies vitro.