Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4) exhibits growth inhibitory effects in prostate cancer cells

摘要: The gene Par-4 (Prostate Apoptosis Response 4) was originally identified in prostate cancer cells undergoing apoptosis and its product showed specific pro-apoptotic activity. Particularly, the SAC domain of (SAC-Par-4) selectively kills leaving normal unaffected. therapeutic significance bioactive SAC-Par-4 is enormous biology; however, large scale production still a matter concern. Here we report SAC-Par-4-GFP fusion protein coupled to translational enhancer sequence (5′ AMV) apoplast signal peptide (aTP) transgenic Nicotiana tabacum cv. Samsun NN plants under control unique recombinant promoter M24. Transgene integration confirmed by genomic DNA PCR, Southern Northern blotting, Real-time PCR Nuclear run-on assays. Results Western blot analysis ELISA expression it as high 0.15% total soluble protein. In addition, found that targeting plant endoplasmic reticulum (ER) essential for stability comparison bacterial derived SAC-Par-4. Deglycosylation demonstrated ER-targeted SAC-Par-4-GFP-SEKDEL undergoes O-linked glycosylation unlike apoplast-targeted SAC-Par-4-GFP. Furthermore, various vitro studies like mammalian proliferation assay (MTT), induction assays, NF-κB suppression suggested cytotoxic apoptotic properties plant-derived against multiple cell lines. Additionally, pre-treatment MAT-LyLu with purified significantly delayed onset tumor syngeneic rat model. Taken altogether, proclaim made may become useful alternate therapy effectively alleviating new era.